1. General

T-2 toxin is a mycotoxin produced by various Fusarium. It mainly pollutes wheat, barley, corn and

other food crops and their products, which constitute a great hazard to human health and animal

husbandry. T2 mainly affects the function of the blood, liver, kidney, pancreatic muscle and

lymphocyte. The clinical symptoms of T2 toxin poisoning is anorexia, vomiting, diarrhea, growth

retardation, reproductive and neurological dysfunction etc.The use of Trichothecenes(T2) ELISA

test kit to analyze T2 residue of samples is fast and accurate.

This kit is a new generation of fungal toxins detection products in application of ELISA technology

and the operation time is shorter than 60min, to minimize operating errors and work intensity.

2. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of T2 in the sample. The coupling antigens are pre-coated on the micro-well stripes. The T2 in the sample and the

coupling antigens pre-coated on the micro-well stripes compete for the anti-T2 antibodies. After the

addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density

(OD) value of the sample has a negative correlation with the T2 in it. This value is compared to the

standard curve and the T2 concentration is subsequently obtained.

3. Application

This test kit can be used to detect T2 in corn, rice, wheat, beans, peanuts, oats, feed and other

samples qualitatively and quantitatively.

4. Technical specifications

Sensitivity: 0.2 ppb

Detection limit:

Peanuts, corn, oats, beans, feed etc. …………………………………………………24ppb

Wheat……………………………………………………………………………………..20ppb

Recovery rate:

110±15%

Coefficient of variation:<10%

5. Components

1) Micro-well strips: 12 strips with 8 removable wells each

2) 6 x Concentrated standards………………………………………………………1ml each

0 ppb (zero standard), 2 ppb, 6 ppb, 18 ppb, 54 ppb, 162 ppb

Standard working concentration: 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb

3) T2 Enzyme conjugate ……………………………………………………………… 6ml

4) T2 Antibody working solution……………………………………………………… 6 ml

5) Substrate A....................................................................................................... 6ml

6) Substrate B....................................................................................................... 6ml

7) Stop solution......................................................................................................6ml

8) Concentrated washin