1. Principle
The test kit is based on the competitive enzyme immunoassay for the detection of Ractopamine in the sample. The conjugated antigens is pre-coated on the micro-well stripes. The Ractopamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Ractopamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Ractopamine concentration in the sample. The value is compared to the standard curve and the Ractopamine concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Incubator temperature: 25℃
Incubator time: 30min~15min
Detection limit
Urine.......................................................................................... 0.05 ppb
Tissue……………………………………………………………………… 0.2 ppb
Meat........................................................................................... 0.05 ppb
Liver............................................................................................. 0.1 ppb
Feed............................................................................................ 0.5 ppb
Cross-reactions
Ractopamine................................................................................... 100%
Dobutamine........................................................................................ 1%
Salbutamol.....................................................................................<0.1%
Clenbuterol.....................................................................................<0.1%
Recovery rate
Urine............................................................................................ 95±18%
Tissue.......................................................................................... 75±21%
Feed............................................................................................ 80±25%
3. Components