1. Principle

The test kit is based on the competitive enzyme immunoassay for the detection of Salbutamol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Salbutamol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Salbutamol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Salbutamol in it. This value is compared to the standard curve and the Salbutamol residues is subsequently obtained.

2. Technical specifications

Sensitivity: 0.1ppb

Detection limit

Tissue(First method)..................................................................... 0.4 ppb

Tissue(Second method)................................................................ 0.1 ppb

Urine, serum................................................................................. 0.1 ppb

Feed............................................................................................... 1 ppb

Recovery rate

Urine, serum................................................................................. 95±10%

Tissue.......................................................................................... 85±15%

Feed............................................................................................ 85±15%

Cross-reaction rate

Salbutamol...................................................................................... 100%

Terbutalin......................................................................................... < 1%

Clenbuterol...................................................................................... < 1%

Ractopamine.................................................................................... < 1%

3. Components

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb

3） Enzyme conjugate (7 mL)............................................................... red cap

4）