1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Clenbuterol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Clenbuterol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Clenbuterol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Clenbuterol in the sample. This value is compared to the standard curve and the Clenbuterol residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit
Urine............................................................................................ 0.1 ppb
Tissue (method one)..................................................................... 0.4 ppb
Tissue (method two)...................................................................... 0.1 ppb
Feed............................................................................................... 1 ppb
Recovery rate
Urine............................................................................................ 95±22%
Tissue.......................................................................................... 75±20%
Feed............................................................................................ 80±20%
Cross-reaction rate
Clenbuterol...................................................................................... 100%
Terbutalin..........................................................................................<1%
Mabuterol ........................................................................................<1%
Brombuterol......................................................................................<1%
Salbutamal....................................................................................... < 1%
Ractopamine.................................................................................... < 1%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard