1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Ampicillin in the sample. The antigens conjugated Ampicillin is pre-coated on the micro-well stripes, Ampicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Ampicillin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Ampicillin concentration in the sample. This value is compared to the standard curve and concentration of Ampicillin in the sample is subsequently obtained.
2. Technical specifications
Sensitivity : 0.1 ppb
Detection limit:
Chicken, duck, pork,liver, fish, shrimp................................................. 2 ppb
Honey, milk....................................................................................... 2 ppb
Because of some interference in honey and milk, the detection limit is 4 ppb.
Recovery rate:
Chicken, duck, pork, liver, fish, shrimp.......................................... 75%±10%
Honey, milk................................................................................. 70%±10%
Cross-reaction rate:
Ampicillin........................................................................................... 100%
Benzyl penicillin.................................................................................. 0.8%
Cloxacillin........................................................................................... 0.2%
Dicloxacillin........................................................................................ 0.1%
Amoxicillin.......................................................................................... 0.1%
Ceftiofu.............................................................................................. 0.1%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7ppb and 8.1 ppb