1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Benzyl penicillin in the chicken, pork, duck, honey, milk, fish, shrimp and egg, etc. The antigens conjugated Benzyl penicillin is pre-coated on the micro-well stripes, Benzyl penicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Benzyl penicillin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Benzyl penicillin concentration in the sample. This value is compared to the standard curve and concentration of Benzyl penicillin in the sample is subsequently obtained.

2. Technical specifications

Sensitivity : 0.05 ppb

Detection limit:

Chicken, duck, pork, liver, fish, shrimp................................................ 1 ppb

Honey, milk....................................................................................... 1 ppb

Recovery rate:

Chicken, duck, pork,liver, fish, shrimp.............................................. 85±10%

Honey, milk.................................................................................... 70±10%

Cross-reaction rate:

Benzyl penicillin................................................................................. 100%

Ampicillin........................................................................................... 0.8%

Cloxacillin........................................................................................... 0.2%

Dicloxacillin........................................................................................ 0.1%

Amoxicillin.......................................................................................... 0.1%

Ceftiofu.............................................................................................. 0.1%

3. Components

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb

3） Enzyme conjugate (12 mL)..................................