1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Diazepam in the feed, urine, liver and meat. The coupling antigen is pre-coated on the micro-well stripes. The Diazepam in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Diazepam antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Diazepam in it. This value is compared to the standard curve and the Diazepam concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.1 ppb

Detection limit

Tissue............................................................................................. 1 ppb

Urine............................................................................................... 1 ppb

Feed............................................................................................. 10 ppb

Recovery rate

Urine........................................................................................... 80 ±10%

Feed........................................................................................... 75 ±10%

Tissue(meat, liver)........................................................................ 85 ±10%

Cross-reactions rate

Diazepam........................................................................................ 100%

Nitrazepam....................................................................................... 7.6%

Oxazepam........................................................................................ 8.8%

3. Components

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb

3） Enzyme conjugate (12 mL)............................................................. red cap

4） Antibody working solution (7 mL).................................................. blue cap