1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Diazepam in the feed, urine, liver and meat. The coupling antigen is pre-coated on the micro-well stripes. The Diazepam in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Diazepam antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Diazepam in it. This value is compared to the standard curve and the Diazepam concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Detection limit
Tissue............................................................................................. 1 ppb
Urine............................................................................................... 1 ppb
Feed............................................................................................. 10 ppb
Recovery rate
Urine........................................................................................... 80 ±10%
Feed........................................................................................... 75 ±10%
Tissue(meat, liver)........................................................................ 85 ±10%
Cross-reactions rate
Diazepam........................................................................................ 100%
Nitrazepam....................................................................................... 7.6%
Oxazepam........................................................................................ 8.8%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb
3) Enzyme conjugate (12 mL)............................................................. red cap
4) Antibody working solution (7 mL).................................................. blue cap