1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Gentamicin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Gentamicin in the sample and pre-coated coupling antigen on the micro-well stripes compete for the anti-Gentamicin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Gentamicin in it. The value is compared to the standard curve and the Gentamicin concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.1 ppb

Detection limit

Chicken............................................................................................ 4 ppb

Liver................................................................................................. 4 ppb

Recovery rate

Chicken......................................................................................... 70±10%

Liver.............................................................................................. 70±10%

Cross-reaction rate

Gentamicin........................................................................................ 100%

Streptomycin......................................................................................<1%

Dihydrostreptomycin...........................................................................<1%

Neomycin...........................................................................................<1%

3. Components

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb

3） Enzyme conjugate (7 mL)............................................................... red cap