1. Principle

The BIOKAU^?β-agonists test kit is based on the competitive enzyme immunoassay for the detection of β-agonists in the urine, pork and other samples. The coupling antigen is pre-coated on the micro-well stripes. The β-agonists in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-β-agonists antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the β-agonists in the sample. This value is compared to the standard curve and the β-agonists residues is subsequently obtained.

2. Technical specifications

Sensitivity: 0.3 ppb

Detection limit:

Tissue.......................................................................................... 0.3 ppb

Urine, serum................................................................................. 0.3 ppb

Feed............................................................................................. 30 ppb

Recovery rate:

Urine, serum................................................................................. 90±10%

Tissue.......................................................................................... 80±10%

Feed............................................................................................ 80±15%

Cross-reaction rate:

Clenbuterol...................................................................................... 100%

Salbutamol...................................................................................... 120%

Bromchlorbuterol............................................................................. 130%

Brombuterol...................................................................................... 90%

Mabuterol.......................................................................................... 70%

Clenpenterol...................................................................................... 65%

Mapenterol........................................................................................ 55%

Tebutalin........................................................................................... 42%

Carbuterol......................................................................................... 35%

Clenproperol..................................................................................... 15%