1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Aminohydantion (AHD) in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Aminohydantion (AHD) in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AHD antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AHD in it. This value is compared to the standard curve and the AHD concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.05 ppb

Detection limit

Tissue(Chicken, duck, porcine meat, beef and mutton)···················· 0.1 ppb

Liver(Chicken, duck, porcine meat, beef and mutton)······················· 0.1 ppb

Milk, intestine, honey····································································· 0.1 ppb

Fish, shrimp (some interference) ·················································· 0.15ppb

Recovery rate

Tissue, liver·················································································· 90±15%

Honey, milk, intestine···································································· 80±15%

Cross-reaction rate

AHD·······························································································&middo