1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Detection limit:
Intestine......................................................................................... 0.1 ppb
Honey.......................................................................................... 0.15 ppb
Milk.............................................................................................. 0.05 ppb
Urine, serum................................................................................... 0.1 ppb
Chicken/liver, pork/liver, fish, shrimp............................................... 0.05 ppb
Recovery rate
Chicken/liver, pork/liver, fish, shrimp................................................ 80±10%
Honey............................................................................................ 70±10%
Milk................................................................................................ 85±15%
Cross-reaction rate:
Chloramphenicol................................................................................ 100%
Thiamphenicol................................................................................. < 0.1%
Florfeniol......................................................................................... < 0.1%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb