1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AMOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AMOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AMOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AMOZ in it. This value is compared to the standard curve and the AMOZ concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Detection limit
Tissue(Chicken, duck, porcine meat, beef and mutton).................... 0.2 ppb
Liver(Chicken, duck, porcine meat, beef and mutton)...................... 0.2 ppb
Honey, milk, intestine, urine........................................................... 0.2 ppb
Fish, shrimp(some interference)..................................................... 0.3 ppb
Recovery rate
Tissue, liver.................................................................................. 90±15%
Honey, milk, intestine.................................................................... 80±15%
Cross-reaction rate
AMOZ............................................................................................. 100%
AHD.............................................................................................<0.01%
AOZ.............................................................................................<0.01%
SEM............................................................................................<0.01%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3)="">