1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Tetracyclines in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tetracyclines in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tetracyclines antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tetracyclines in it. This value is compared to the standard curve and the Tetracyclines concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.05 ppb

Detection limit:

Chicken,duck, pork(meat or liver), shrimp, fish, milk

Tetracyclines..................................................................................... 1ppb

Minocycline...................................................................................... 1ppb

RoliTetracyclines............................................................................... 1ppb

Aureomycin...................................................................................... 1ppb

Demethylchlor Tetracyclines............................................................... 3ppb

Terramycin........................................................................................ 2ppb

Doxycycline...................................................................................... 3ppb

Honey

Tetracyclines..................................................................................... 2ppb

Minocycline...................................................................................... 2ppb

RoliTetracyclines............................................................................... 2ppb

Aureomycin...................................................................................... 2ppb

Demethylchlor Tetracyclines............................................................... 6ppb

Terramycin........................................................................................ 4ppb

Doxycycline...................................................................................... 6ppb

Cross-reaction rate:

Tetracyclines.................................................................................... 100%

Minocycline...................................................................................... 125%

RoliTetracyclines............................................................................... 110%

Aureomycin...................................................................................... 100%