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Mouse Cortisol ELISA Kit
Model Number:PDT00537E
Place of Origin:China
Added:2015-05-06
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Keywords:Cortisol
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Product Details

Product Name:

Mouse Cortisol ELISA Kit

Product Type :ELISA Kit
Code :PDT00537E
Size :96T,5×96T,10×96T
Abbreviation :COR
Protein Biological Process 1 :Metabolism
Species :Mouse
Sample Types :serum, plasma and tissue homogenates
Detect Range :0.049 ng/ml-200 ng/ml
Sensitivity :0.049 ng/ml
Assay Time :1-5h
Sample Volume :50-100ul
Detection Wavelengt :450 nm
Availability :3-5 working days
Research Area :Metabolism
Protocol :
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%


Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%


Three samples of known concentration were tested in twenty assays to assess.








Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of mouse cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

SampleSerum(n=4)
1:1Average %93
Range %86-97
1:2Average %97
Range %92-103
1:4Average %89
Range %85-92
1:8Average %90
Range %84-96
Recovery :
The recovery of mouse cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9088-93
EDTA plasma (n=4)9490-98