| Product Name: | Mouse Cortisol ELISA Kit |
| Product Type : | ELISA Kit |
| Code : | PDT00537E |
| Size : | 96T,5×96T,10×96T |
| Abbreviation : | COR |
| Protein Biological Process 1 : | Metabolism |
| Species : | Mouse |
| Sample Types : | serum, plasma and tissue homogenates |
| Detect Range : | 0.049 ng/ml-200 ng/ml |
| Sensitivity : | 0.049 ng/ml |
| Assay Time : | 1-5h |
| Sample Volume : | 50-100ul |
| Detection Wavelengt : | 450 nm |
| Availability : | 3-5 working days |
| Research Area : | Metabolism |
| Protocol : | |
| Precision : | | Intra-assay Precision (Precision within an assay): CV%<8% |
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| | Three samples of known concentration were tested twenty times on one plate to assess. |
| | Inter-assay Precision (Precision between assays): CV%<10% |
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| | Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity : | | To assess the linearity of the assay, samples were spiked with high concentrations of mouse cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) |
| | 1:1 | Average % | 93 |
| | Range % | 86-97 |
| | 1:2 | Average % | 97 |
| | Range % | 92-103 |
| | 1:4 | Average % | 89 |
| | Range % | 85-92 |
| | 1:8 | Average % | 90 |
| | Range % | 84-96 |
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| Recovery : | | The recovery of mouse cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range |
| | Serum (n=5) | 90 | 88-93 |
| | EDTA plasma (n=4) | 94 | 90-98 |
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