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Chicken Vascular Endothelial cell Growth Factor,VEGF ELISA KIT
Model Number:PDT02978E
Place of Origin:CHINA
Keywords:Vascular Endothelial cell Growth Factor,VEGF
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Product Details

Product Name:

Chicken Vascular Endothelial cell Growth Factor A(VEGF-A) ELISA KIT

Product Type :ELISA Kit
Code :PDT02978E
Size :96T,5×96T,10×96T
Uniprot NO. :P67964
Abbreviation :VEGFA
Protein Biological Process 1 :Angiogenesis
Alias :RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor
Species :Chicken
Protein Biological Process 3 :Angiogenesis
Sample Types :serum, plasma, tissue homogenates
Detect Range :15.6 pg/ml-1000 pg/ml
Sensitivity :3.9 pg/ml
Assay Time :1-5h
Sample Volume :50-100ul
Detection Wavelengt :450 nm
Availability :3-5 working days
Research Area :Cancer
Protocol :
Protocol may be improved. Please feel free to contact us to obtain the latest version.
Target details :This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of chicken VEGF-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

1:1Average %92
Range %89-108
1:2Average %89
Range %82-96
1:4Average %95
Range %90-104
1:8Average %95
Range %86-98
Recovery :
The recovery of chicken VEGF-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9389-100
EDTA plasma (n=4)9589-101