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Porcine endothelin 1,ET-1 ELISA Kit
Model Number:PDT00643E
Place of Origin:CHINA
Added:2015-05-06
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Keywords:endothelin 1,ET-1
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Product Details

Product Name:

Porcine endothelin 1,ET-1 ELISA Kit

Product Type :ELISA Kit
Code :PDT00643E
Size :96T,5×96T,10×96T
Uniprot NO. :P09558
Abbreviation :EDN1
Alias :ET1, HDLCQ7, preproendothelin 1
Species :Pig
Sample Types :serum, plasma and tissue homogenates
Detect Range :3.12 pg/ml-200pg/ml
Sensitivity :0.78pg/ml
Assay Time :1-5h
Sample Volume :50-100ul
Detection Wavelengt :450 nm
Availability :3-5 working days
Research Area :Cancer


Target details :This protein is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.
HGNC :3176
RGD :2532
MGI :95283
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%


Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%


Three samples of known concentration were tested in twenty assays to assess.








Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of pig ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

SampleSerum(n=4)
1:1Average %100
Range %94-105
1:2Average %97
Range %92-101
1:4Average %89
Range %85-93
1:8Average %95
Range %91-101
Recovery :
The recovery of pig ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9085-94
EDTA plasma (n=4)8480-89