| Product Name: | Porcine endothelin 1,ET-1 ELISA Kit |
| Product Type : | ELISA Kit |
| Code : | PDT00643E |
| Size : | 96T,5×96T,10×96T |
| Uniprot NO. : | P09558 |
| Abbreviation : | EDN1 |
| Alias : | ET1, HDLCQ7, preproendothelin 1 |
| Species : | Pig |
| Sample Types : | serum, plasma and tissue homogenates |
| Detect Range : | 3.12 pg/ml-200pg/ml |
| Sensitivity : | 0.78pg/ml |
| Assay Time : | 1-5h |
| Sample Volume : | 50-100ul |
| Detection Wavelengt : | 450 nm |
| Availability : | 3-5 working days |
| Research Area : | Cancer |
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| Target details : | This protein is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. |
| HGNC : | 3176 |
| RGD : | 2532 |
| MGI : | 95283 |
| Precision : | | Intra-assay Precision (Precision within an assay): CV%<8% |
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| | Three samples of known concentration were tested twenty times on one plate to assess. |
| | Inter-assay Precision (Precision between assays): CV%<10% |
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| | Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity : | | To assess the linearity of the assay, samples were spiked with high concentrations of pig ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) |
| | 1:1 | Average % | 100 |
| | Range % | 94-105 |
| | 1:2 | Average % | 97 |
| | Range % | 92-101 |
| | 1:4 | Average % | 89 |
| | Range % | 85-93 |
| | 1:8 | Average % | 95 |
| | Range % | 91-101 |
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| Recovery : | | The recovery of pig ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range |
| | Serum (n=5) | 90 | 85-94 |
| | EDTA plasma (n=4) | 84 | 80-89 |
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