Product Name: | Porcine endothelin 1,ET-1 ELISA Kit |
Product Type : | ELISA Kit |
Code : | PDT00643E |
Size : | 96T,5×96T,10×96T |
Uniprot NO. : | P09558 |
Abbreviation : | EDN1 |
Alias : | ET1, HDLCQ7, preproendothelin 1 |
Species : | Pig |
Sample Types : | serum, plasma and tissue homogenates |
Detect Range : | 3.12 pg/ml-200pg/ml |
Sensitivity : | 0.78pg/ml |
Assay Time : | 1-5h |
Sample Volume : | 50-100ul |
Detection Wavelengt : | 450 nm |
Availability : | 3-5 working days |
Research Area : | Cancer |
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Target details : | This protein is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. |
HGNC : | 3176 |
RGD : | 2532 |
MGI : | 95283 |
Precision : | Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity : | To assess the linearity of the assay, samples were spiked with high concentrations of pig ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) |
| 1:1 | Average % | 100 |
| Range % | 94-105 |
| 1:2 | Average % | 97 |
| Range % | 92-101 |
| 1:4 | Average % | 89 |
| Range % | 85-93 |
| 1:8 | Average % | 95 |
| Range % | 91-101 |
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Recovery : | The recovery of pig ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
Sample Type | Average % Recovery | Range |
| Serum (n=5) | 90 | 85-94 |
| EDTA plasma (n=4) | 84 | 80-89 |
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