1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Fumonisin B1 in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Fumonisin B1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Fumonisin B1 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Fumonisin B1 concentration in the sample. This value is compared to the standard curve and the Fumonisin B1 concentration is subsequently obtained.

2. Technical specifications

Sensitivity : 10 ppb

Detection limit:

Grain, corn, feed.......................................................................... 0.5 ppm

Linear range:

10ng/mL~200ng/mL

Recovery rate:

Grain, corn, feed........................................................................... 90±15%

Precision:
Intra-assay CV<10%;
Inter-assay CV<15%

3. Components

1） Micro-well strips:96wells(12 strips with 8 removable wells each)

2） 5× standard solution (0.5 mL each): 0 ppb,10 ppb, 50 ppb, 100 ppb,200 ppb

3） Anti-FB1 monoclonal antibody solution(6ml)............... ready for use directly

4） Enzyme conjugate (12 mL)......................................... ready for use directly

5） Substrate solution (12 mL)......................................... ready for use directly

6） Stop solution (6 mL)....................