1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained.

2. Technical specifications

Sensitivity : 5 ppb

Detection limit:

Milk................................................................................................. 5 ppb

Tissue(chicken, pork, duck, fish, shrimp and liver)............................ 10 ppb

Feed............................................................................................ 500 ppb

Egg............................................................................................. 100 ppb

Recovery rate:

Milk.............................................................................................. 90±15%

Tissue.......................................................................................... 85±10%

Feed............................................................................................ 85±10%

Egg.............................................................................................. 80±10%

Cross-reaction rate:

Melamine......................................................................................... 100%

Cyanuric acid..................................................................................... 60%

Triazine..............................................................................................<1%

Diamino atrazine................................................................................<1%