| Product Name: | Human Angiotensin Ⅱ Receptor 2 antibody,AT2R-Ab ELISA Kit |
| Product Type : | ELISA Kit |
| Code : | PDT03376E |
| Size : | 96T,5×96T,10×96T |
| Abbreviation : | AT2R-Ab |
| Species : | Human |
| Sample Types : | serum, plasma |
| Detect Range : | 0.312 U/ml-20 U/ml |
| Sensitivity : | 0.078 U/ml |
| Assay Time : | 1-5h |
| Sample Volume : | 50-100ul |
| Detection Wavelengt : | 450 nm |
| Availability : | 3-5 working days |
| Research Area : | Cardiovascular |
| Protocol : | |
| Precision : | | Intra-assay Precision (Precision within an assay): CV%<8% |
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| | Three samples of known concentration were tested twenty times on one plate to assess. |
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| | Inter-assay Precision (Precision between assays): CV%<10% |
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| | Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity : | | To assess the linearity of the assay, samples were spiked with high concentrations of human AT2R-Ab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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| Sample | Serum(n=4) |
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| | 1:1 | Average % | 95 |
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| | Range % | 82-105 |
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| | 1:2 | Average % | 94 |
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| | Range % | 84-100 |
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| | 1:4 | Average % | 98 |
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| | Range % | 92-101 |
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| | 1:8 | Average % | 94 |
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| | Range % | 82-105 |
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| Recovery : | | The recovery of human AT2R-Ab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| | Sample Type | Average % Recovery | Range |
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| | Serum (n=5) | 95 | 83-105 |
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| | EDTA plasma (n=4) | 95 | 85-103 |
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