Product Name: | Goat Interferon γ ,IFN-γ ELISA Kit |
Product Type : | ELISA Kit |
Code : | PDT04061E |
Size : | 96T,5×96T,10×96T |
Uniprot NO. : | P79154 |
Abbreviation : | IFNG |
Protein Biological Process 1 : | Immunity |
Alias : | IFG, IFI, |
Species : | Goat |
Protein Biological Process 3 : | Antiviral defense |
Sample Types : | serum, plasma, tissue homogenates |
Detect Range : | 7.8 pg/ml-500 pg/ml |
Sensitivity : | 1.95 pg/ml |
Assay Time : | 1-5h |
Sample Volume : | 50-100ul |
Detection Wavelengt : | 450 nm |
Availability : | 3-5 working days |
Research Area : | Immunology |
Protocol : | Protocol may be improved. Please feel free to contact to obtain the latest version. |
HGNC : | 5438 |
RGD : | 2866 |
MGI : | 107656 |
Precision : | Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity : | To assess the linearity of the assay, samples were spiked with high concentrations of goat IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) |
| 1:1 | Average % | 92 |
| Range % | 87-96 |
| 1:2 | Average % | 103 |
| Range % | 99-107 |
| 1:4 | Average % | 93 |
| Range % | 89-98 |
| 1:8 | Average % | 92 |
| Range % | 89-94 |
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Recovery : | The recovery of goat IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
Sample Type | Average % Recovery | Range |
| Serum (n=5) | 97 | 93-102 |
| EDTA plasma (n=4) | 91 | 88-95 |
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