| Product Name: | Goat Interferon γ ,IFN-γ ELISA Kit |
| Product Type : | ELISA Kit |
| Code : | PDT04061E |
| Size : | 96T,5×96T,10×96T |
| Uniprot NO. : | P79154 |
| Abbreviation : | IFNG |
| Protein Biological Process 1 : | Immunity |
| Alias : | IFG, IFI, |
| Species : | Goat |
| Protein Biological Process 3 : | Antiviral defense |
| Sample Types : | serum, plasma, tissue homogenates |
| Detect Range : | 7.8 pg/ml-500 pg/ml |
| Sensitivity : | 1.95 pg/ml |
| Assay Time : | 1-5h |
| Sample Volume : | 50-100ul |
| Detection Wavelengt : | 450 nm |
| Availability : | 3-5 working days |
| Research Area : | Immunology |
| Protocol : | Protocol may be improved. Please feel free to contact to obtain the latest version. |
| HGNC : | 5438 |
| RGD : | 2866 |
| MGI : | 107656 |
| Precision : | | Intra-assay Precision (Precision within an assay): CV%<8% |
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| | Three samples of known concentration were tested twenty times on one plate to assess. |
| | Inter-assay Precision (Precision between assays): CV%<10% |
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| | Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity : | | To assess the linearity of the assay, samples were spiked with high concentrations of goat IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) |
| | 1:1 | Average % | 92 |
| | Range % | 87-96 |
| | 1:2 | Average % | 103 |
| | Range % | 99-107 |
| | 1:4 | Average % | 93 |
| | Range % | 89-98 |
| | 1:8 | Average % | 92 |
| | Range % | 89-94 |
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| Recovery : | | The recovery of goat IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range |
| | Serum (n=5) | 97 | 93-102 |
| | EDTA plasma (n=4) | 91 | 88-95 |
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