1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Streptomycin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Streptomycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti- Streptomycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Streptomycin concentration in the sample. This value is compared to the standard curve and the Streptomycin concentration is subsequently obtained.

2. Technical specifications

Sensitivity : 0.1 ppb

Detection limit:

Chicken, Chicken liver, Milk............................................................... 4 ppb

Honey, Royal jelly............................................................................ 2 ppb

Recovery rate:

Milk.............................................................................................. 95±15%

Chicken........................................................................................ 85±10%

Honey, Royal jelly......................................................................... 75±15%

Cross-reaction rate:

Streptomycin.................................................................................... 100%

Dihydrostreptomycin........................................................................ 108%

Kalamycin..........................................................................................<1%

Gentamycin.......................................................................................<1%

3. Components

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.4 ppb, 1.6 ppb, 6.4 ppb, 25.6 ppb