1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Florfenicol in the tissue (chicken, pork), fish, shrimp and honey. The coupling antigens are pre-coated on the micro-well stripes. The Florfenicol in the sample and the conjugate antigens pre-coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value has a negative correlation with the Florfenicol concentration in the sample. This value is compared to the standard curve and the Florfenicol concentration is subsequently obtained.
2. Technical specifications
Sensitivity : 0.5 ppb
Detection limit:
Honey........................................................................................... 1.5 ppb
Meat, liver, shrimp, fish.................................................................. 0.5 ppb
Recovery rate:
Honey......................................................................................... 70 ±10%
Meat, liver, shrimp, fish................................................................ 80 ±10%
Cross-reaction rate:
Florfenicol........................................................................................ 100%
Florfenicol Amine............................................................................... 11%
Thiamphenicol................................................................................ < 0.1%
Chloramphenicol............................................................................ < 0.1%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb
3) Enzyme conjugate (12 mL)............................................................. red cap
4) Concentrated Antibody working solution (1 mL).............................