1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Sulfonamides in the chicken, pork, milk, honey, egg, etc. The coupling antigens are pre-coated on the micro-well stripes. The Sulfonamides in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfonamides antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfonamides in the sample. This value is compared to the standard curve and the Sulfonamides concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1 ppb
Detection limit
Tissue............................................................................................. 1 ppb
Serum, urine.................................................................................... 4 ppb
Milk............................................................................................... 20 ppb
Recovery rate
Tissue....................................................................................... 70 ±10%
Egg.......................................................................................... 65 ±10%
Milk, honey, serum..................................................................... 70 ±10%
Cross-reaction rate
Sulfadiazine (SD or SDZ)................................................................. 100%
Sulfamonomethoxine (SMM)........................................................... 97.6%
Sulfamerazine (SM1)........................................................................... 12%